Start with a pump out. Flush the holding tank with fresh water (blast some down the vent line if you can while the pump out is running. ) The suction may help evacuate the line from the toilet.
When you use the toilet pump, does it feel rock hard, or kinda springy? If it is spongy/springy, that means that there is a blockage in the line. More stiff, then it is a mechanical issue with the pump.
Unfortunately, if there is still effluent in the line, when you remove the pump it will spill some, have kitty litter ready as an absorbent. And get a gallon jug of Raritan k.o. to use in the cleanup efforts. Plugged toilets are always a pain, and rarely a clean job.
One easy thing to check however is the flush control valve. Make sure it is all the way in either wet or dry. If the valve is stuck half way, it will block the flow and act like a plugged line.