You NEED a biocide ......
Bacterial and fungal growth in diesel fuels is a well known and established fact. The 'problem' is that although when cracked and distilled at super high tempedratures which yields a very dehydrated fluid, shipment, storage etc. allows 'moisture uptake' to occur by chemical equilibrium. The principal species of contamination in fuel oil (regardless of sulphur content) is pseudomonas aeroginosa (bacteria) and Cladosporium resinae (fungus). Essentially they are 'activated' in fuel oil in the presence of WATER. Water in fuel oil is in TWO forms: free water, that which eventually falls to the bottom of a tank by gravimetric settling; emulsified water - that which is held in equilibrium 'between the molecules of the fuel'. These bio-species in the presence of WATER, use the fuel (carbon) as their nutrient sources, and then by cell division - multiply. Their products of decomposition (when they 'die') is what forms the 'sludge' and the 'resin' that adheres to the tank walls, etc. So, you either need a means to continually remove the two forms of water from the fuel, prevent the water from being 'equilibrated' into the fuel OR need a biocide to prevent/retard the growth. The longer the fuel is in contact with the ATMOSPHERE (the VENT on your tank) the more the need for separation or biocide. Free Water can be removed by periodically draining it from the tank (nearly impossible to do on a 'boat tank' - as CG RULES prevent a tank 'bottom drain') or by 'catching it' in the separator section of a Racor, etc. filter (with integral separator) or by the use of a 'water blocking filter media' very uncommon in (boat) fuel oil filters. Emulsified water usually only is removed by large filters that contain a 'water absorbing starch' (hydroxymethyl-cellulose, etc.). OR a desiccant filter is added to the VENT to prevent the moisture 'uptake' (by chemical equilibrium) .... but that only removes water to -40deg. 'dewpoint'. ... since by 'common means' its virtually impossible to keep fuel oil (exposed to the atmosphere) water free .... YOU NEED BIOCIDE, especially if you store the fuel 'long term'. ANY diesel fuel compatible BIOCIDE will do. A tank that is full will absorb MORE water than a tank that is nearly empty .... its all governed by chemical 'equilibrium'. If the tank has a VENT, it WILL 'uptake' water. Filling a tank to prevent 'condensation' is simply BS. ANY fuel oil compatible BIOCIDE will do. I run a 'sophisticated' tank system .... it includes a desiccant filter and a 2,5µM particle filter capsule on the VENT .... lessens the water uptake and filters out the fungal spores. Plus I run an onboard polishing system with an integral (free) water knockout. pot