or snake oil.You guys must love living dangerously!
I'm not certain what you would call "conclusive proof."
We know that:
Microbes can live in the interface of water and diesel fuel. That's a lot of black crud you get in the tank.
Microbes live and die and leave their little bodies to accumulate as a sludge.
Some microbes produce very acidic waste products, think microbe pee.
Aluminum reacts poorly to acid.
Pick up tubes in fuel tanks do not reach the bottom of the tank, so by the time they pick up the sludge, there is a lot of it.
The aviation industry is very concerned about microbes in jet fuel. Jet fuel and diesel are not chemically that different.
Murphy says that the filter will not clog with dead microbes when the boat is safely at the dock.
Water gets into the fuel tank in various ways, sometimes through the tank vent, sometimes through failed filler gaskets.
Water is heavier than diesel so it settles to the bottom of the tank where it is not evacuated by the fuel pump until there is way too much water.
I think those are enough reasons to keep a fuel tank full and to use a biocide. However, your boat, your life, just think of the folks who would have to effect a rescue as your fuel filter clogs on a lee shore.
Using an additive is cheap insurance.
You can say all you want about how it kills microbes in a petri dish, but I would like to see evidence of how it works in the real world.