An inability to pump much out of the tank, or put water into the tank is almost certain to indicate a blocked tank vent. An earlier indication is a manual toilet that's becoming increasingly harder to pump or an electric toilet that's begun to "burp" or back up flushes. Don't use the toilet again or attempt to pumpout again until the vent is clear.
Running some water backwards through the vent isn't that easy because all "vent" thru-hulls are designed for use on fuel tanks...so they're caps with pinholes in it or just a slit in the bottom...or they have screens in 'em. I don't recommend taking the vent line off to inspect, because a vent blockage is likely to pressurize the system, which will result in a spew.
90% of vent blockages occur in the vent thru-hull...so start by using an ice pick, screwdriver blade or whatever works to dislodge and clean out anything that's clogged it. Screens become clogged with rust, dust and pollen...if there is one, knock it out...screens cause more problems than they solve.
If that doesn't solve the problem, check the vent line for any kinks or sags where liquid may have pooled in it. If you don't find any, the blockage is almost certain be at the tank end of the vent line--in that end of the hose and the vent fitting on the tank. You will have to remove the vent line to clean it out. But BEFORE you do that, open the deck pumpout cap to relieve any pressure in the system! Now you won't put half a tankful of waste into your bilge if you take a hose off.
Warming a hose a bit with a blow dryer will make it a lot easier to get off the fitting. Scrape out that end of the hose and the vent fitting on the tank. Unless you've overfilled the tank enough to push undissolved TP into the vent line, that should open it up. Warm the hose again and use a little dishwashing liquid to lubricate it to put it back on.
But as long as you've done all this, now would be a great time to replace the vent thru-hull with one that you can stick a hose nozzle up against and backflush the vent line to keep it clear every time you wash the boat and/or pump out...'cuz as is pretty obvious from reading what you have to do to cure a blocked vent, preventing it is LOT easier! Use a plain ol' open "bulkhead" thru-hull (see photo)..5/8" is the standard size. Backflushing it will take care of any insects who find their way into it.
You might want to check out the link in my signature...it goes to complete "toilets and holding tanks 101" guide to installing, operating and maintaining the sanitation system and can prevent a LOT of "head aches!"