I would recommend launching in goodland, driving thru marco island.(951)
not us41 to (92) . cr 92 road is very bumpy, and 41 is 2 lanes... not advised in the mac, take alligator alley. (75) 4 lanes divided w/ rest stops.
http://www.calusaislandmarina.com/
http://maps.google.com/maps?q=calus...817,-81.640091&spn=0.151285,0.220757&t=h&z=12
http://maps.google.com/maps?q=calus...755,-81.651608&spn=0.001182,0.001725&t=h&z=19
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I have launched in everglades city, on the barron river,at a trailer park, that closed, that was a good ramp. I'm not sure if it has reopened. there maybe another ramp you could use, but may need to go around cokolooskee, and thats a very tricky route, even at high tide.
I also launched at the (everglades city) rod and gun club, *(they have a tree over the ramp, so you you need to raise the stick IN the water) - a motel. -the rod and gun club is a old 1900's winter hunting lodge, built of wood, mostly with cypress. sits right on the river w/ large screened porches. lunch there is very cool. but the river runs at 2-3 knts, so you don't want to fight the tide, and its an hour run from indian key. but they do have fuel.
http://www.evergladesrodandgun.com/
flamingo is accessed thru the main park entrance, via miami/homestead. think that's 70miles from the park entrance to the ramps at flamingo. follow the speed limit.. those rangers wait all day for speeders... not much there but bugs, small snack bar, don't thing the hotel is back open, but there is camping room. when you get to the ramp. good ramp, but shallow. watch there are 2 sides, bay and ocean (they have a sling lift to move skiffs across). be sure to launch on the right side...
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looking at the everglades city chamber of commerce, looks like barron river resort is now: everglades isle...(equity rv park) just sent an email to ask about use of their ramp...
-update: the answer is no public access... use goodland!
(everglades city: