Ethylene glycol (automotive) anti-freeze is toxic... non-toxic propylene glycol marine/rv antifreeze ("the pink stuff") is, as the name implies, not. If it were it could not be used in potable water systems, which it often is.\Plus using POISONOUSAntifreeze around any thing that people can use (sink, galley, heads) is not recommended.
Jim...
The same mfr also recommends using ethylene glycol antifreeze--which is very destructive to the rubber parts in a toilet.You dump antifreeze into the bowl and pump it dry through the waste hoses and valves to the holding tank. Then you disconnect the waste hoses from the pump, and open the bowl bottom drain. NO antifreeze is used in a Jabsco manual toilet pump - that is the manufacturer's instruction. Instead you drain it down and open it up at the bottom waste connection.
Much simpler to either disconnect the toilet intake line from the thru-hull (close the seacock first!), stick into a jug of the "pink stuff" and pump the whole gallon all the way through to the tank...or, if your toilet inlet line is teed into the head sink drain line, close the seacock on the head sink drain thru-hull, pour "the pink stuff" down the sink and flush it all the way through to the tank. That is, AFTER you've not only pumped out the tank but THOROUGHLY rinsed it out.